Determine Disinfectant and Antiseptic Effectiveness Using Zone Inhibition Method

Lab Report Title Page countersink Disinfectant and Antiseptic Effectiveness Using Zone Inhibition manner Prepared for By Date Introduction Page Abstract This content is to examine the effects of different types of disinfectants by disk diffusion mode using common agents such as Betadine, Clorox, Crest, Kiss My Face, Listerine, and Lysol. Wound dispense and general cleaning of home surfaces is a part of daily act for many of the general population. Knowing which agents are effective once morest the bacteria that close to commonly cause infection, disease, and illness all(a)ows educated decisions on which agents to use.We compared the bactericidal metier of 6 common agents widely used by the general worldly concern against 3 common bacterial organisms. Introduction Sterilization crop ups all organisms in or on an object or substance. Disinfectants and nonpurulents do not set up since many types of organisms and spores are not killed. Disinfectants are described as antimic robic agents that are used on inanimate objects such as an shaft or structural surfaces. The term antiseptic is usually applied to healthful agents that are used on living tissue such as skin and throat mucosa.A disinfectant must be assailable of killing pathogens enchantment it is in contact with them, so that they cannot grow again when it is removed. This action is express to be cidal (lethal), and it is described according to the type of organisms it kills as bactericidal, sporicidal, fungicidal, virucidal, etc If the antimicrobic substance merely inhibits the organisms while it is in contact with them, they whitethorn be able to multiply again when it is removed. In this case, the agent is said to have static activity and may be described as bacteriostatic, fungistatic, etcMicroorganisms of different groups are not uniformly susceptible to chemical disinfection due(p) to the structures of their cell walls. As an example, Mycobacterium are more resistant than to the upl iftedest degree other bacteria because of their waxy cell walls. Of all microbial forms, bacterial endospores display the greatest resistance to both chemical and physical agents of disinfection. The talent claimed for a given disinfectant in killing vegetative bacterial species may have no bearing on its ability to degrade bacterial or fungal spores, mycobacteria, some viruses or prions.A public figure of factors must be considered when choosing a disinfectant besides type of organism. These embarrass exposure time, concentration of the antimicrobic agent, temperature, optimal pH activity of the antimicrobic agents, the concentration of the microorganisms present, and the toxicity of the agent for skin or its effect on materials to be disinfected. Here we testament test the effectiveness of respective(a) antimicrobial substances by inoculating culture plates with the test organisms and then(prenominal) applying the disinfectant/antiseptic to the inoculated plate by a filt er paper disk that has been dip into the disinfectant/antiseptic.This is a disk diffusion regularity that is similar to the method used to test the effectiveness of various(a) chemotherapeutic agents. We will then measure the order-of-inhibition. With this method, the presence of such a clear zone (lack of growth) touch the chemical shows either the cells have been killed or that their growth has been inhibited. A zone of inhibition does not discriminate between bacterio-static and bactericidal chemicals. The 6 agents we will be interrogatory are Betadine (B) A povidone-iodine dressing used in the first place for skin disinfection and wound treatment.Kills a wide range of disease-causing organisms including bacteria, fungi, many viruses, and nearly micro-organisms. Does not affect hydrophilic viruses such as polyomavirus and PBFD. Clorox (C) A biguanide preparation of Sodium Hypochlorite used mainly for surface cleaning in various environments such as homes and hospitals to kill microorganisms. Bleach is effective as a disinfectant and kills many micro-organisms, but is not a viable termination to the spread of tuberculosis, bacterial spores and Chlamydia. Crest (Cr) A widely used antimicrobial for oral bacteria and biofilms.Especially useful on Gram-negative micro-organisms such as Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens, and Fusobacterium nucleatum. Kiss My Face (Kf) An thorough alternative to typical intoxicant based mouthwashes using aloe vera as antimicrobial agent and grapefruit aspirate as a polyphenol. Proven useful with Trichophyton mentagrophytes and Staphylococcus aureus. _ _. Listerine (Li) An alcohol based antiseptic mouth rinse containing the active ingredient fermentation alcohol which is toxic to bacteria at concentrations of only 7%. Reduces dental carries, plaque and gingivitis.Lysol (Ly) A O-phenylphenol preparation of cresols used for surface cleaning in various environments suc h as homes and hospitals to kill microorganisms such as fungi, Tuberculosis, certain viruses,_ staphylococcus_ and genus Pseudomonas bacteria. Does not kill nonenveloped viruses, such as _parvovirus, polyomavirus, papillomavirus, adenovirus and reovirus_, nor will they kill all bacteria types. The collar common bacteria we will be testing against are Pseudomonas aeruginosa An ubiquitous environmental organism associated with whirlpools whereby infection is caused by immersion in contaminated water.Staphylococcus aureus A universal industrial plant organism on the skin surfaces of most healthy humans. Escherichia coli A large(p) and diverse group of organisms associated with fecal remnants that have been ingested causing infection. Materials and Methods Page Materials and Methods T-Soy plates (TSA) Lawn spread head method Sterile swabs Forceps Sterile filter paper disks Disinfectants/Antiseptics We will go on the specific instructions to carry out this experiment as celebr ated in Lab 16 p90 of Symbiosis by Pearson. Results Page Results and banterThis experiment was conducted to show how different disinfectants and antiseptics kill bacteria. The results of our experiment under normal room conditions on the TSA agar were as follows final result Page Conclusion Previous research has shown that if an antiseptic disinfectant agent does not kill bacteria, the risk of infection is importantly higher. We used a Gram-negative (P aeruginosa), a Gram-positive (S aureus), and a Gram-negative (_E. coli_) bacterium for bactericidal testing procedures. We allowed the TSA agar to mature over a 7 day period and then noted our results by viewing and measuring the zone inhibition (mm).Findings were that while all had an effect, there were differences among how effective each agent was against each physique of bacterium used. Surface cleaning agents Lysol was most effective against S. aureus and E. Coli, while Clorox did significantly well with all triplet bacteri um. It appears from our findings that Clorox is the most effective disinfectant and antimicrobial of the six agents tested. Oral care agents The effects of Listerine were less than stealer, while the organic product Kiss My Face was more effective with all three types of bacteria. Crest rated low as an effective agent for all three types of bacteria.It is important to note that the standard deviation rose as high as 7. 8, which is a significant difference in the resulting data accuracy. literary works Cited Pearson, 2010. Symbiosis Lab 16 Disk Diffusion Assay to run across Disinfectant and Antiseptic Effectiveness, pp. 89-91. Pearson Custom Publishing Vicki S. Rabenberg, Christopher D. Ingersoll, Michelle A. Sandrey, and Mary T. Johnson. The Bactericidal And cytotoxic Effects Of Antimicrobial Wound Cleansers. 2002. Centers for Disease Control and Prevention http//www. cdc. gov/nczved/divisions/dfbmd/diseases/ecoli_o157h7/index. hypertext markup languagespread Violet I. Haraszthy, DDS, MS, PhD Joseph J.Zambon, DDS, PhDPrem K. Sreenivasan, PhD. The Antimicrobial Efficacy of Commercial Dentifrices Featured in General Dentistry. 2010 Agarry O. , Olaleye M. T, and Bello-Michael, C. O. Comparative antimicrobial activities of aloe vera gel and leaf. African Journal of biotech Vol. 4 (12), pp. 1413-1414, 2005. ZDENKA CVETNI, ANDA VLADIMIR-KNE. Antimicrobial activity of grapefruit seed and pulp ethanolic extract. discussion section of Microbiology Faculty of pharmacy and BiochemistryUniversity of Zagreb in conjunction with the Croatia and Department of Pharmacognosy Faculty of Pharmacy and Biochemistry University of Zagreb, Croatia. 2004.